Stability and antioxidant capacity of epigallocatechin gallate in Dulbecco's modified eagle medium
نویسندگان
چکیده
Though the instability of polyphenols in cell culture experiment has been investigated previously, underlying mechanism is not completely clear yet. Therefore, this study, stability epigallocatechin gallate (EGCG) medium DMEM was at 4 °C and 37 via UPLC-MS-MS analysis followed by determination antioxidant capacity EGCG. EGCG instable formed various degradation products derived from its dimer with increasing incubation time many isomers being both temperatures. The were more stable than °C. structure formation five analyzed four unidentified. Ascorbic acid significantly improved protecting auto-oxidation DMEM, particularly antioxidative activity determined DPPH, ABTS FRAP assay. properties continuously decreased over 8 h which consistent course degradation.
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ژورنال
عنوان ژورنال: Food Chemistry
سال: 2022
ISSN: ['1873-7072', '0308-8146']
DOI: https://doi.org/10.1016/j.foodchem.2021.130521